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addgene 43802  (Addgene inc)


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    Addgene inc addgene 43802
    Addgene 43802, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+plasmid/p414-TEF1p-Cas9-CYC1t+(Plasmid+%2343802)/pmc12856427-1-10-10
    Average 93 stars, based on 127 article reviews
    addgene 43802 - by Bioz Stars, 2026-09
    93/100 stars

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    Recombinant:

    Article Title: Selector adeno-associated viral vectors facilitate on-target precise genome editing and purge off-target chromosomal insertions.
    Article Snippet: Reagent or Resource Source Identifier Chemicals, peptides, and recom binant proteins Penicillin/streptomycin Thermo Fisher Scientific Cat#15140-122 Non-essential amino acids Thermo Fisher Scientific Cat#11140-050 GlutaMax supplement Thermo Fisher Scientific Cat#35050-061 Recombinant Human Fibroblast Growth Factor-basic Peprotech Cat#100-18B Dexamethasone Sigma-Aldrich Cat#D2915 Polyethylenimine (PEI) Polysciences Cat#23966-1 PEG 8000 Sigma-Aldrich Cat#P2139 Benzonase Millipore Cat#E1014-25KU Lodixanol-OptiPrep Progen Cat#1114542 Poloxamer 188 Sigma-Aldrich Cat#P5556 α-thioglycerol Sigma-Aldrich Cat#M6145 Bathocuprione disulphonate Sigma-Aldrich Cat#B1125 Ouabain Sigma-Aldrich Cat#03125 Trypsin-EDTA Thermo Fisher Scientific Cat#15400-054 Critical commercial assays Qubit dsDNA HS assay kit Thermo Fisher Scientific Cat#Q32854 Agilent DNA 1000 reagent kit Agilent Cat#5067-1504 Phusion High-Fidelity Polymerase Thermo Fisher Scientific Cat#F-530L AMPure XP beads Beckman Coulter Cat#A63881 iQ SYBR Green Supermix Bio-Rad Cat#L010171C Phire Tissue Direct PCR Master mix Thermo Fisher Scientific Cat# F170L DNeasy Blood & Tissue kit QIAGEN Cat#69506 Deposited da ta NGS raw data This paper PRJNA1373510 Experimental models: Cell lines HeLa ATCC RRID:CVCL_0030 Human mesenchymal stem cells Gonçalves et al [50] NA HEK293T ATCC RRID:CVCL_0063 Human muscle progenitor cells (AB1098) Mamchaoui et al [51]. .. Thorley et al [52] NA Oligonucleotides Primers in Table S6, S8, S10, S14, S17 This paper NA Recombinant DNA BI17_pAAV-HRS1.A1 This paper Supplementary information BI19_pAAV-HRLMN.A1 This paper Supplementary information BI38_pAAV-HR.RA1.IN17 This paper Supplementary information BM42_pAAV-HR.GA1.IN17 This paper Supplementary information (continued on next page) Trends in Biotechnology, Month 2026, Vol. xx, No. xx 19 (c 2 ontinued) Reagent or Resource 0 Trends in Biotechnology, Month 2026, Vol. xx, No. xx Source Identifier BJ62_pAAV-HR.V2LMN.A1 This paper Supplementary information BM51_pAAV-HRNPM1 This paper Supplementary information LMNA_mScarlet-I_Donor Levesque et al [16] Addgene Plasmid #178092 NPM1_mNeonGreen_Donor Levesque et al [16] Addgene Plasmid #178093 ATP1A1_plasmid_donor_RD Agudelo et al [13] Addgene Plasmid #86551 ATP1A1_T804N_hPGK1_mScarlet-I_Donor Levesque et al [16] Addgene Plasmid #173207 AT51_pDG6.RSV.DsRed.SV40pA Grimm et al [53]. .. NA Software and algorithms FlowJo 10.9.0 FlowJo https://www.flowjo.com/ GraphPad Prism 9.3.1 GraphPad Prism https://www.graphpad.com/scientific-software/prism/ CRISPResso2 Clement et al [54]. https://github.com/pinellolab/CRISPResso2 Other Quick-Seal round-top polypropylene tube Beckman Cat#342414 Amicon Ultra-15 100K MWCO filter Millipore Cat#UFC910024

    Article Title: RNF25 confers mRNA damage tolerance by curbing activation of the integrated stress response.
    Article Snippet: MS-5 mouse cells DSMZ ACC 441 .. Oligonucleotides Oligonucleotide sequences used in this study are provided in Table S9 This study N/A Recombinant DNA pcDNA5/FRT/TO-RNF25-WT-mVenus3XFlag This study N/A pcDNA5/FRT/TO-RNF25-ΔRWD-mVenus3XFlag This study N/A pcDNA5/FRT/TO-RNF25-ΔRING-mVenus3XFlag This study N/A pcDNA5/FRT/TO-mVenus-3xFlagGateway Di Fiore and Pines80 Addgene Plasmid #40999 pOG44_FLP_recombinase Hallacli et al.81 Addgene Plasmid #209087 Genome-wide All-in-One Brunello lentiviral library Doench et al.82 Addgene pooled library #73179 pX330-Puro Pritchard et al.83 Addgene Plasmid #82580 pU6-tevopreq1-GG-acceptor vector Nelson et al.84 Addgene Plasmid #174038 pU6-pegRNA-GG-acceptor vector Anzalone et al.85 Addgene Plasmid #132777 pCMV-PEmax Chen et al.86 Addgene Plasmid #174820 pEF1a-hMLH1dn Chen et al.86 Addgene Plasmid #174824 lentiGuide-Puro Sanjana et al.87 Addgene Plasmid #52963 pMDLg/pRRE Dull et al.88 Addgene Plasmid #12251 pRSV-Rev Dull et al.88 Addgene Plasmid #12253 pMD2.G/pVSV-g Didier Trono Addgene Plasmid #12259 Software and algorithms ImageLab (Version 5.2) Bio-Rad RRID:SCR_014210 SparkControl 3.1 Tecan https://lifesciences.tecan.com/multimode- plate-reader?p=tab–3 R (version 4.3.2) R Project for Statistical Computing RRID:SCR_001905 GraphPad Prism (Version 9.1.0) Dotmatics RRID:SCR_002798 R package limma (v. 3.58.1) Bioconductor RRID:SCR_010943 MaxQuant (v.1.5.2.8) MaxQuant RRID:SCR_014485 PhosphoSitePlus Kinase Library Johnson et al.61 https://kinase-library.phosphosite.org Snapgene (Version 5.0.8) GSL Biotech LLC RRID:SCR_015052 R package preprocessCore (Version 1.64.0) Bioconductor https://bioconductor.org/packages/ release/bioc/html/preprocessCore.html cutadapt (version see respective methods section) Martin89 https://cutadapt.readthedocs.io; RRID:SCR_011841 Bowtie2 (version 2.5.0) Langmead and Salzberg90 http://bowtie-bio.sourceforge.net/bowtie2/ index.shtml; RRID:SCR_016368 Samtools Li et al.91 http://samtools.sourceforge.net/; RRID:SCR_002105 STAR (version see respective methods section) Dobin et al.92 https://github.com/alexdobin/STAR; RRID:SCR_004463 Scikit-ribo, modified version Fang et al.93 Gao et al.94 https://github.com/nedialkova-lab/scikitribo-ext (Continued on next page) e4 Molecular Cell 86, 1275–1292.e1–e12, April 2, 2026 ..

    Plasmid Preparation:

    Article Title: Selector adeno-associated viral vectors facilitate on-target precise genome editing and purge off-target chromosomal insertions.
    Article Snippet: Reagent or Resource Source Identifier Chemicals, peptides, and recom binant proteins Penicillin/streptomycin Thermo Fisher Scientific Cat#15140-122 Non-essential amino acids Thermo Fisher Scientific Cat#11140-050 GlutaMax supplement Thermo Fisher Scientific Cat#35050-061 Recombinant Human Fibroblast Growth Factor-basic Peprotech Cat#100-18B Dexamethasone Sigma-Aldrich Cat#D2915 Polyethylenimine (PEI) Polysciences Cat#23966-1 PEG 8000 Sigma-Aldrich Cat#P2139 Benzonase Millipore Cat#E1014-25KU Lodixanol-OptiPrep Progen Cat#1114542 Poloxamer 188 Sigma-Aldrich Cat#P5556 α-thioglycerol Sigma-Aldrich Cat#M6145 Bathocuprione disulphonate Sigma-Aldrich Cat#B1125 Ouabain Sigma-Aldrich Cat#03125 Trypsin-EDTA Thermo Fisher Scientific Cat#15400-054 Critical commercial assays Qubit dsDNA HS assay kit Thermo Fisher Scientific Cat#Q32854 Agilent DNA 1000 reagent kit Agilent Cat#5067-1504 Phusion High-Fidelity Polymerase Thermo Fisher Scientific Cat#F-530L AMPure XP beads Beckman Coulter Cat#A63881 iQ SYBR Green Supermix Bio-Rad Cat#L010171C Phire Tissue Direct PCR Master mix Thermo Fisher Scientific Cat# F170L DNeasy Blood & Tissue kit QIAGEN Cat#69506 Deposited da ta NGS raw data This paper PRJNA1373510 Experimental models: Cell lines HeLa ATCC RRID:CVCL_0030 Human mesenchymal stem cells Gonçalves et al [50] NA HEK293T ATCC RRID:CVCL_0063 Human muscle progenitor cells (AB1098) Mamchaoui et al [51]. .. Thorley et al [52] NA Oligonucleotides Primers in Table S6, S8, S10, S14, S17 This paper NA Recombinant DNA BI17_pAAV-HRS1.A1 This paper Supplementary information BI19_pAAV-HRLMN.A1 This paper Supplementary information BI38_pAAV-HR.RA1.IN17 This paper Supplementary information BM42_pAAV-HR.GA1.IN17 This paper Supplementary information (continued on next page) Trends in Biotechnology, Month 2026, Vol. xx, No. xx 19 (c 2 ontinued) Reagent or Resource 0 Trends in Biotechnology, Month 2026, Vol. xx, No. xx Source Identifier BJ62_pAAV-HR.V2LMN.A1 This paper Supplementary information BM51_pAAV-HRNPM1 This paper Supplementary information LMNA_mScarlet-I_Donor Levesque et al [16] Addgene Plasmid #178092 NPM1_mNeonGreen_Donor Levesque et al [16] Addgene Plasmid #178093 ATP1A1_plasmid_donor_RD Agudelo et al [13] Addgene Plasmid #86551 ATP1A1_T804N_hPGK1_mScarlet-I_Donor Levesque et al [16] Addgene Plasmid #173207 AT51_pDG6.RSV.DsRed.SV40pA Grimm et al [53]. .. NA Software and algorithms FlowJo 10.9.0 FlowJo https://www.flowjo.com/ GraphPad Prism 9.3.1 GraphPad Prism https://www.graphpad.com/scientific-software/prism/ CRISPResso2 Clement et al [54]. https://github.com/pinellolab/CRISPResso2 Other Quick-Seal round-top polypropylene tube Beckman Cat#342414 Amicon Ultra-15 100K MWCO filter Millipore Cat#UFC910024

    Article Title: RNF25 confers mRNA damage tolerance by curbing activation of the integrated stress response.
    Article Snippet: MS-5 mouse cells DSMZ ACC 441 .. Oligonucleotides Oligonucleotide sequences used in this study are provided in Table S9 This study N/A Recombinant DNA pcDNA5/FRT/TO-RNF25-WT-mVenus3XFlag This study N/A pcDNA5/FRT/TO-RNF25-ΔRWD-mVenus3XFlag This study N/A pcDNA5/FRT/TO-RNF25-ΔRING-mVenus3XFlag This study N/A pcDNA5/FRT/TO-mVenus-3xFlagGateway Di Fiore and Pines80 Addgene Plasmid #40999 pOG44_FLP_recombinase Hallacli et al.81 Addgene Plasmid #209087 Genome-wide All-in-One Brunello lentiviral library Doench et al.82 Addgene pooled library #73179 pX330-Puro Pritchard et al.83 Addgene Plasmid #82580 pU6-tevopreq1-GG-acceptor vector Nelson et al.84 Addgene Plasmid #174038 pU6-pegRNA-GG-acceptor vector Anzalone et al.85 Addgene Plasmid #132777 pCMV-PEmax Chen et al.86 Addgene Plasmid #174820 pEF1a-hMLH1dn Chen et al.86 Addgene Plasmid #174824 lentiGuide-Puro Sanjana et al.87 Addgene Plasmid #52963 pMDLg/pRRE Dull et al.88 Addgene Plasmid #12251 pRSV-Rev Dull et al.88 Addgene Plasmid #12253 pMD2.G/pVSV-g Didier Trono Addgene Plasmid #12259 Software and algorithms ImageLab (Version 5.2) Bio-Rad RRID:SCR_014210 SparkControl 3.1 Tecan https://lifesciences.tecan.com/multimode- plate-reader?p=tab–3 R (version 4.3.2) R Project for Statistical Computing RRID:SCR_001905 GraphPad Prism (Version 9.1.0) Dotmatics RRID:SCR_002798 R package limma (v. 3.58.1) Bioconductor RRID:SCR_010943 MaxQuant (v.1.5.2.8) MaxQuant RRID:SCR_014485 PhosphoSitePlus Kinase Library Johnson et al.61 https://kinase-library.phosphosite.org Snapgene (Version 5.0.8) GSL Biotech LLC RRID:SCR_015052 R package preprocessCore (Version 1.64.0) Bioconductor https://bioconductor.org/packages/ release/bioc/html/preprocessCore.html cutadapt (version see respective methods section) Martin89 https://cutadapt.readthedocs.io; RRID:SCR_011841 Bowtie2 (version 2.5.0) Langmead and Salzberg90 http://bowtie-bio.sourceforge.net/bowtie2/ index.shtml; RRID:SCR_016368 Samtools Li et al.91 http://samtools.sourceforge.net/; RRID:SCR_002105 STAR (version see respective methods section) Dobin et al.92 https://github.com/alexdobin/STAR; RRID:SCR_004463 Scikit-ribo, modified version Fang et al.93 Gao et al.94 https://github.com/nedialkova-lab/scikitribo-ext (Continued on next page) e4 Molecular Cell 86, 1275–1292.e1–e12, April 2, 2026 ..

    Article Title: The human branchpoint-interacting stem-loop sequence and structure regulates U2 snRNA expression, branchpoint recognition, and the transcriptome
    Article Snippet: .. For the orthogonal U2 snRNA construct, the RNU2-1 locus, including its Pol II promoter and 3′ processing site, was amplified from HeLa genomic DNA [ ] and subcloned into the BglII site of Addgene plasmid #44170. ..

    Genome Wide:

    Article Title: RNF25 confers mRNA damage tolerance by curbing activation of the integrated stress response.
    Article Snippet: MS-5 mouse cells DSMZ ACC 441 .. Oligonucleotides Oligonucleotide sequences used in this study are provided in Table S9 This study N/A Recombinant DNA pcDNA5/FRT/TO-RNF25-WT-mVenus3XFlag This study N/A pcDNA5/FRT/TO-RNF25-ΔRWD-mVenus3XFlag This study N/A pcDNA5/FRT/TO-RNF25-ΔRING-mVenus3XFlag This study N/A pcDNA5/FRT/TO-mVenus-3xFlagGateway Di Fiore and Pines80 Addgene Plasmid #40999 pOG44_FLP_recombinase Hallacli et al.81 Addgene Plasmid #209087 Genome-wide All-in-One Brunello lentiviral library Doench et al.82 Addgene pooled library #73179 pX330-Puro Pritchard et al.83 Addgene Plasmid #82580 pU6-tevopreq1-GG-acceptor vector Nelson et al.84 Addgene Plasmid #174038 pU6-pegRNA-GG-acceptor vector Anzalone et al.85 Addgene Plasmid #132777 pCMV-PEmax Chen et al.86 Addgene Plasmid #174820 pEF1a-hMLH1dn Chen et al.86 Addgene Plasmid #174824 lentiGuide-Puro Sanjana et al.87 Addgene Plasmid #52963 pMDLg/pRRE Dull et al.88 Addgene Plasmid #12251 pRSV-Rev Dull et al.88 Addgene Plasmid #12253 pMD2.G/pVSV-g Didier Trono Addgene Plasmid #12259 Software and algorithms ImageLab (Version 5.2) Bio-Rad RRID:SCR_014210 SparkControl 3.1 Tecan https://lifesciences.tecan.com/multimode- plate-reader?p=tab–3 R (version 4.3.2) R Project for Statistical Computing RRID:SCR_001905 GraphPad Prism (Version 9.1.0) Dotmatics RRID:SCR_002798 R package limma (v. 3.58.1) Bioconductor RRID:SCR_010943 MaxQuant (v.1.5.2.8) MaxQuant RRID:SCR_014485 PhosphoSitePlus Kinase Library Johnson et al.61 https://kinase-library.phosphosite.org Snapgene (Version 5.0.8) GSL Biotech LLC RRID:SCR_015052 R package preprocessCore (Version 1.64.0) Bioconductor https://bioconductor.org/packages/ release/bioc/html/preprocessCore.html cutadapt (version see respective methods section) Martin89 https://cutadapt.readthedocs.io; RRID:SCR_011841 Bowtie2 (version 2.5.0) Langmead and Salzberg90 http://bowtie-bio.sourceforge.net/bowtie2/ index.shtml; RRID:SCR_016368 Samtools Li et al.91 http://samtools.sourceforge.net/; RRID:SCR_002105 STAR (version see respective methods section) Dobin et al.92 https://github.com/alexdobin/STAR; RRID:SCR_004463 Scikit-ribo, modified version Fang et al.93 Gao et al.94 https://github.com/nedialkova-lab/scikitribo-ext (Continued on next page) e4 Molecular Cell 86, 1275–1292.e1–e12, April 2, 2026 ..

    Software:

    Article Title: RNF25 confers mRNA damage tolerance by curbing activation of the integrated stress response.
    Article Snippet: MS-5 mouse cells DSMZ ACC 441 .. Oligonucleotides Oligonucleotide sequences used in this study are provided in Table S9 This study N/A Recombinant DNA pcDNA5/FRT/TO-RNF25-WT-mVenus3XFlag This study N/A pcDNA5/FRT/TO-RNF25-ΔRWD-mVenus3XFlag This study N/A pcDNA5/FRT/TO-RNF25-ΔRING-mVenus3XFlag This study N/A pcDNA5/FRT/TO-mVenus-3xFlagGateway Di Fiore and Pines80 Addgene Plasmid #40999 pOG44_FLP_recombinase Hallacli et al.81 Addgene Plasmid #209087 Genome-wide All-in-One Brunello lentiviral library Doench et al.82 Addgene pooled library #73179 pX330-Puro Pritchard et al.83 Addgene Plasmid #82580 pU6-tevopreq1-GG-acceptor vector Nelson et al.84 Addgene Plasmid #174038 pU6-pegRNA-GG-acceptor vector Anzalone et al.85 Addgene Plasmid #132777 pCMV-PEmax Chen et al.86 Addgene Plasmid #174820 pEF1a-hMLH1dn Chen et al.86 Addgene Plasmid #174824 lentiGuide-Puro Sanjana et al.87 Addgene Plasmid #52963 pMDLg/pRRE Dull et al.88 Addgene Plasmid #12251 pRSV-Rev Dull et al.88 Addgene Plasmid #12253 pMD2.G/pVSV-g Didier Trono Addgene Plasmid #12259 Software and algorithms ImageLab (Version 5.2) Bio-Rad RRID:SCR_014210 SparkControl 3.1 Tecan https://lifesciences.tecan.com/multimode- plate-reader?p=tab–3 R (version 4.3.2) R Project for Statistical Computing RRID:SCR_001905 GraphPad Prism (Version 9.1.0) Dotmatics RRID:SCR_002798 R package limma (v. 3.58.1) Bioconductor RRID:SCR_010943 MaxQuant (v.1.5.2.8) MaxQuant RRID:SCR_014485 PhosphoSitePlus Kinase Library Johnson et al.61 https://kinase-library.phosphosite.org Snapgene (Version 5.0.8) GSL Biotech LLC RRID:SCR_015052 R package preprocessCore (Version 1.64.0) Bioconductor https://bioconductor.org/packages/ release/bioc/html/preprocessCore.html cutadapt (version see respective methods section) Martin89 https://cutadapt.readthedocs.io; RRID:SCR_011841 Bowtie2 (version 2.5.0) Langmead and Salzberg90 http://bowtie-bio.sourceforge.net/bowtie2/ index.shtml; RRID:SCR_016368 Samtools Li et al.91 http://samtools.sourceforge.net/; RRID:SCR_002105 STAR (version see respective methods section) Dobin et al.92 https://github.com/alexdobin/STAR; RRID:SCR_004463 Scikit-ribo, modified version Fang et al.93 Gao et al.94 https://github.com/nedialkova-lab/scikitribo-ext (Continued on next page) e4 Molecular Cell 86, 1275–1292.e1–e12, April 2, 2026 ..

    Modification:

    Article Title: RNF25 confers mRNA damage tolerance by curbing activation of the integrated stress response.
    Article Snippet: MS-5 mouse cells DSMZ ACC 441 .. Oligonucleotides Oligonucleotide sequences used in this study are provided in Table S9 This study N/A Recombinant DNA pcDNA5/FRT/TO-RNF25-WT-mVenus3XFlag This study N/A pcDNA5/FRT/TO-RNF25-ΔRWD-mVenus3XFlag This study N/A pcDNA5/FRT/TO-RNF25-ΔRING-mVenus3XFlag This study N/A pcDNA5/FRT/TO-mVenus-3xFlagGateway Di Fiore and Pines80 Addgene Plasmid #40999 pOG44_FLP_recombinase Hallacli et al.81 Addgene Plasmid #209087 Genome-wide All-in-One Brunello lentiviral library Doench et al.82 Addgene pooled library #73179 pX330-Puro Pritchard et al.83 Addgene Plasmid #82580 pU6-tevopreq1-GG-acceptor vector Nelson et al.84 Addgene Plasmid #174038 pU6-pegRNA-GG-acceptor vector Anzalone et al.85 Addgene Plasmid #132777 pCMV-PEmax Chen et al.86 Addgene Plasmid #174820 pEF1a-hMLH1dn Chen et al.86 Addgene Plasmid #174824 lentiGuide-Puro Sanjana et al.87 Addgene Plasmid #52963 pMDLg/pRRE Dull et al.88 Addgene Plasmid #12251 pRSV-Rev Dull et al.88 Addgene Plasmid #12253 pMD2.G/pVSV-g Didier Trono Addgene Plasmid #12259 Software and algorithms ImageLab (Version 5.2) Bio-Rad RRID:SCR_014210 SparkControl 3.1 Tecan https://lifesciences.tecan.com/multimode- plate-reader?p=tab–3 R (version 4.3.2) R Project for Statistical Computing RRID:SCR_001905 GraphPad Prism (Version 9.1.0) Dotmatics RRID:SCR_002798 R package limma (v. 3.58.1) Bioconductor RRID:SCR_010943 MaxQuant (v.1.5.2.8) MaxQuant RRID:SCR_014485 PhosphoSitePlus Kinase Library Johnson et al.61 https://kinase-library.phosphosite.org Snapgene (Version 5.0.8) GSL Biotech LLC RRID:SCR_015052 R package preprocessCore (Version 1.64.0) Bioconductor https://bioconductor.org/packages/ release/bioc/html/preprocessCore.html cutadapt (version see respective methods section) Martin89 https://cutadapt.readthedocs.io; RRID:SCR_011841 Bowtie2 (version 2.5.0) Langmead and Salzberg90 http://bowtie-bio.sourceforge.net/bowtie2/ index.shtml; RRID:SCR_016368 Samtools Li et al.91 http://samtools.sourceforge.net/; RRID:SCR_002105 STAR (version see respective methods section) Dobin et al.92 https://github.com/alexdobin/STAR; RRID:SCR_004463 Scikit-ribo, modified version Fang et al.93 Gao et al.94 https://github.com/nedialkova-lab/scikitribo-ext (Continued on next page) e4 Molecular Cell 86, 1275–1292.e1–e12, April 2, 2026 ..

    Construct:

    Article Title: The human branchpoint-interacting stem-loop sequence and structure regulates U2 snRNA expression, branchpoint recognition, and the transcriptome
    Article Snippet: .. For the orthogonal U2 snRNA construct, the RNU2-1 locus, including its Pol II promoter and 3′ processing site, was amplified from HeLa genomic DNA [ ] and subcloned into the BglII site of Addgene plasmid #44170. ..

    Amplification:

    Article Title: The human branchpoint-interacting stem-loop sequence and structure regulates U2 snRNA expression, branchpoint recognition, and the transcriptome
    Article Snippet: .. For the orthogonal U2 snRNA construct, the RNU2-1 locus, including its Pol II promoter and 3′ processing site, was amplified from HeLa genomic DNA [ ] and subcloned into the BglII site of Addgene plasmid #44170. ..



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